All-in-One™ miRNA qRT-PCR Detection Kit and validated miRNA primers

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GeneCopoeia
Product
All-in-One™ miRNA qRT-PCR Detection Kit and validated miRNA primers
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Features

• Sensitive and practical quantitative profiling of miRNA expression



• Detects only mature miRNA



• Two-step detection and quantification method of multiple miRNAs from one cDNA synthesis.



• Combines PCR technology and SYBR® Green: fast and accurate quantitative analysis of mature human miRNAs from total RNA samples as small as 100 pg



• Novel optimized blend of poly A polymerase and reverse transcriptase with an RT buffer specifically developed for the All-in-One Reverse Transcriptase Mix: delivers maximum activity of both enzymes for robust and reproducible data.



• 700 validated miRNA primers available

Fig. 1. Overview of steps involved in the All-in-One miRNA qRT-PCR Detection Kit. During 3' polyadenylation, M-MLV RTase and a unique Oligo dT Adaptor primer, reverse transcribes the poly A miRNAs. The kit qRT-PCR Mix, containing SYBR® Green specifically detects the reverse transcribed miRNAs.

The All-in-One qPCR miRNA-specific primers are designed by a proprietary algorithm and validated for more than 700 human miRNAs. When used in combination with the All-in-One SYBR® Green miRNA qRT-PCR kit, the All-in-One miRNA primers deliver reliable and reproducible high performance in quantitative PCR assays.







Fig. 2. All-in-One miRNA qPCR pimers are validated to generate a single amplification of the correct size for the targeted miRNA and to yield a single dissociation curve peak. A cDNA pool, containing reverse transcribed products from 10 human tissue total RNA samples was used as the validation template. qPCR was performed using 0.2 uM primer with 2X All-in-One qPCR Mix. (2A) validated result for amplification curve (2B) validated result for melting curve.

• high-performance gene-expression profiling human miRNAs.
• Combine with our validated mature miRNA-specific primers: more than 700 human miRNA primers.
• Design experiments with our precursor miRNA plasmid constructs and assays for comprehensive functional analysis studies.
• Follow-up with functional validation studies using our ORF expression-ready clones.
• See also:

• miExpress™ precursor miRNA expression clones
• miTarget miRNA target sequence 3' UTR clones
• Luc-Pair™ miR Luciferase Assays

1. Cat No AOMD-Q020, All-in-One miRNA qRT-PCR Detection Kit (20 RT and 200 qPCR reactions): 395 €

Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers; miRNA-specific primers sold separately.

2. Cat No AOMD-Q050, All-in-One miRNA qRT-PCR Detection Kit (50 RT and 500 qPCR reactions): 780 €

Poly A Polymerase, RTase Mix, qPCR Mix, ROX Reference Dye, Universal Adaptor PCR Primer and other buffers; miRNA-specific primers sold separately.

3. For validated specific primers (118 €): Identify the miRNA of interest:

You can browse the entire Pimers list
and use the website search tool to find the primer matching the mature miRNA of interest; Or gather as much as possible of the following information: Precursor/Mature miRNA Name, Precursor/Mature miRNA Accession, Description or Catalog/product ID.
You can also submit a sequence so we can search by Precursor or Mature Sequences homology (BLAST) and we will do the rest.

Alternatively, you can use the search tool by GeneCoppoeia - Search miRNA clones and qPCR primers by GeneCopoeia - and order the product ID result.