Bio-Adembeads Streptavidin 300nm 0,5ml

Supplier
Ademtech
Cat. No
2003130
Kit size
0,5 ml
Links
ademtech Protocol Information Sheet Binding capacities

Features

• Perfect spherical shape with an important surface area: up to 15 m2/g
• Available in 200nm, 300nm (or 500nm see MasterBeads Streptavidin of 500nm).
• Unique size distribution
• High binding capacity: >600pmol/mg
• Many applications: immunoassays, protein purification, cell sorting or molecular biology purification (such as mRNA isolation)

Bio-Adembeads Streptavidin are monodispersed and superparamagnetic particles coated with streptavidin. They are produced under asceptic conditions and are supplied at 0,5% (5mg/ml) and resuspended in Storage Buffer (#2010201),an aqueous suspension containing 0.05% Proclin 300. Streptavidin is a 60,000 dalton protein isolated from the microorganism Streptomyces avidinii and composed of four identical subunits, each of which has a binding site for biotin. Bio-Adembeads Streptavidin are used for binding biotinylated ligands such as oligonucleotides, nucleic acids or proteins. Their high iron oxide content associated with the powerful streptavidin-biotin interaction, make Bio-Adembeads Streptavidin a very efficient tool for separation assays.

Simply wash the beads, resuspend in immobilization buffer and then immobilize the biotinylated molecule at room temp for 30 minutes.

Diameter	300 nm (CV max 20%)
Density	approx. 2.0g/cm3
Magnetisation at saturation	approx. 40emu/g
Specific surface area	15m2/g
Iron oxide content	approx. 70%
Solid content	5mg/ml

Bio-Adembeads Streptavidin are used for binding biotinylated ligands such as oligonucleotides, nucleic acids or proteins. Their high iron oxide content associated with the powerful streptavidin-biotin interaction, make Bio- Adembeads Streptavidin a very efficient tool for separation assays. Immunoassays, protein purification, cell sorting or molecular biology purification