Carboxy-H2DCFDA ROS indicator
| Supplier |
|---|
| GeneCopoeia |
| Cat. no. |
| C264 |
| Kit size |
| 25 mg |
| Links |
Protocol  |
Features
- Fast and easy method
- Fluorescence reading
- Live cell assay
Generation of reactive oxygen species (ROS) at a controlled level is a feature of live cell function. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in damage of membrane lipids, proteins, and nucleic acids. Oxidative damage is associated with aging as well as many diseases. Probes for measuring ROS provide important tools for study oxidative stress related diseases.
Carboxy-H2DCFDA [5-(and 6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate] is a chemically reduced analogue of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent Carboxy-H2DCFDA is converted to the highly fluorescent 5-(and 6)-carboxy-2',7'-dichlorofluorescein, with additional negative charges that impede its leakage out of the cell.
.| Format | 25 mg |
|---|---|
| Fluorescence reading | 495/529 nm |
- Detection of oxidative activities in viable cells
- Probe for ROS