Cell Lysis Reagent

Supplier
Norgen Biotek
Cat. no.
1018801
Size
500 mL
Links
Norgen Biotek

Principle

Cell Lysis Reagent is a proprietary solution of detergent, protease-inhibitor and buffer for the efficient lysis of bacterial cells and extraction of inclusion body proteins.

Cell lysis is accomplished through non-ionic detergent chemical disruption. Passing cells through a needle (not provided) assists in mechanical disruption and reduces viscosity, producing protein often >95% pure. Cell Lysis Reagent does not solubilize inclusion bodies. Inclusion Bodies may be solubilized using Norgen's Inclusion Body Solubilization Reagent (available separately). Also Available: Inclusion Body Isolation Kit.

Efficient Isolation of Inclusion Body Proteins Using Norgen Cell Lysis Reagent and Inclusion Body Solubilization Reagent.

Inclusion Bodies were extracted and solubilized following gene expression. Recovered proteins were analyzed on 12% SDS-PAGE and stained with Coomassie Brilliant Blue R-250. Numbers represent kDa sizes of protein bands