DHE [Dihydroethidium] Superoxide indicator
| Supplier |
|---|
| GeneCopoeia |
| Cat. no. |
| C260 |
| Kit size |
| 25 mg |
| Links |
Protocol  |
Features
- Fast and easy method
- Fluorescence reading
- Live cell assay
Generation of reactive oxygen species (ROS) at a controlled level is a feature of live cell function. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in damage of membrane lipids, proteins, and nucleic acids. Oxidative damage is associated with aging as well as many diseases. Probes for measuring ROS provide important tools for study oxidative stress related diseases.
Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (Ex/Em: 355/420nm) in cells, while the oxidized form ethidium has red fluorescence (Ex/Em: 300/610 nm) upon DNA intercalation.
.| Format | 25 mg |
|---|---|
| Fluorescence reading | 300/610 nm |
- Detection of oxidative activities in viable cells
- including respiratory burst in phagocytes