PrepGEM Universal Genomic DNA extraction-Kit (1000 rxns)

In stock
SKU
PUN01000
1 950,00 €

 PrepGEM Universal Genomic DNA extraction-Kit

 

Overview

Cat. No
PUN1000
Kit size
1000 preps

Features

  • Single-tube extraction
  • Ultra fast: Protocols ranging from 4 to 18 minutes
  • High DNA Recovery – Minimal loss of DNA during extraction
  • No magnetic beads – No spin-columns – No harsh chemicals
  • Protocols scalable from 1 cell to millions of cells
  • Easily automated on a standard laboratory thermocycler or robotic platform
  • Minimal plasticware required – Reduced waste

Principle

The PrepGEM Universal Genomic DNA extraction-Kit is a new system which is specially designed for the capture of gDNA and direct amplification. It utilizes MicroGEM’s thermophilic proteinase for simple, rapid DNA extraction with unprecedented recovery. Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture . The PrepGEM Universal Genomic DNA extraction-Kit is an excellent tool for researchers performing confirmatory genotyping of transfected cells following CRISPR/Cas9 gene editing. Researchers can work with fewer cells - even single cell - and still get enough DNA for downstream analysis. Results are much faster. This method also makes this Kit ideal for processing numerous samples (e..g. 96 well plates) where maximun DNA recovery is critical. Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing. Perfect for CRISPR/Cas9 gene editing with NGS

Sample Preparation

1.From cells: : Prepare sample. Mix sample and reagents (prepGEM and buffers). Place in a standard thermocycler for 2-10 minutes at 75°C. Denature prepGEM enzymes at 95°C for 2 minutes
2.From animal tissues: Procedure described up to 2mg: Add 79μl of DNA-free water, 10 µl of 10X Orange+ buffer, 1 µl PrepGem and 10μl of Histosolv to a thin-walled PCR tube. Add the sample. Mash the sample with a pipette tip and disperse by vortexing. In a thermal cycler, incubate 5 min at 52°C, 10 min at 75°C and 5 min at 95°C. Aspirate the extract away from residual material.

Contents

PrepGem Enzyme
Buffers BLUE Buffer RED+ Buffer Orange+ Buffer Histosolv
Storage Store at 2-8°C.

Applications

  • For DNA extraction from cells, tissue samples, blood, swabs (saliva, etc.), saliva or blood on storage cards , insects, mouse tails
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