Target clones

Genome-wide human, mouse and rat microRNA (miRNA) 3' UTR target clones in mammalian expression vectors. miRNA 3' UTR target clones can be used for miRNA target identification and functional validation of predicted targets or to study the regulatory effect of miRNA on target gene(s).

• 3' UTR sequences were obtained from public domain gene sequence databases and inserted downstream of the secreted Gaussia luciferase (GLuc) reporter gene in its respective vector system, driven by SV40 promoter for expression in mammalian cells. A chimeric mRNA is transcribed consisting of the GLuc and a 3' UTR target sequence. Thus, the study of mRNA-miRNA target interaction can be easily performed with a live cell assay for GLuc using only 10 μl of the cell culture medium.

• Besides using GLuc as the miRNA 3' UTR target reporter, a secreted Alkaline Phosphatase (SEAP) reporter driven by a CMV promoter, is also cloned into the same vector (pEZX-MT05) and serves as the internal control. The dual-reporter vector system enables transfection-normalization for accurate across-sample comparison.

• In addition to the GLuc/SEAP dual-reporter vector system, a firefly/Renilla Duo-Luciferase reporter vector (pEZX-MT06) is also available.