Cas9 stable cell lines
Stable integration at the Human AAVS1 Safe Harbor site ensures consistent, stable expression of the CRISPR-Cas9 nuclease. Cas9 expression from this locus exhibits high activity, as shown by the T7 Endonuclease I mismatch cleavage assay (Figure 2).
Figure 2. Demonstration of stable Cas9 activity in a cell line. Human H1299 cells stably expressing Cas9 nuclease from the AAVS1 Safe Harbor site were transfected with a plasmid carrying an sgRNA for the HUWE I gene. 24 hours post transfection, cells were harvested and used for genomic DNA extraction. Using the GeneCopoeia IndelCheck™ kit, PCR products flanking the sgRNA target site were generated, denatured, reannealed, and treated with (+) or without (-) T7 Endonuclease I, which cleaves mismatched DNA. The presence of two bands below the uncut band at the correct sizes indicates that Cas9 is active in these cells.
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Human cell line LOVO stably expressing CRISPR Cas9, randomly inserted, single clone1 550,00 €
Human cell line LOVO stably expressing CRISPR Cas9, randomly inserted, single clone (indicated price is for Academic; Industrial customers please inquire)
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Human cell line SNU-1 stably expressing Cas9, randomly inserted, single clone1 550,00 €
Human cell line SNU-1 stably expressing Cas9, randomly inserted, single clone (indicated price is for Academic; Industrial customers please inquire)
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Human cell line KATOIII stably expressing CRISPR Cas9, randomly inserted, single clone1 550,00 €
Human cell line KATOIII stably expressing CRISPR Cas9, randomly inserted, single clone (indicated price is for Academic; Industrial customers please inquire)
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Human cell line PLC/PRF/5 stably expressing Cas9, randomly inserted, single clone1 550,00 €
Human cell line PLC/PRF/5 stably expressing Cas9, randomly inserted, single clone (indicated price is for Academic; Industrial customers please inquire)
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Human cell line SNU-449 stably expressing Cas9, randomly inserted, single clone1 550,00 €
Human cell line SNU-449 stably expressing Cas9, randomly inserted, single clone (indicated price is for Academic; Industrial customers please inquire)
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Human cell line LS411N stably expressing Cas9, randomly inserted, single clone1 550,00 €
Human cell line LS411N stably expressing Cas9, randomly inserted, single clone (indicated price is for Academic; Industrial customers please inquire)
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Rat cell line C6 stably expressing Cas9, randomly inserted, single clone1 550,00 €
Rat cell line C6 stably expressing Cas9, randomly inserted, single clone (indicated price is for Academic; Industrial customers please inquire)
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Human cell line SNU-423 stably expressing Cas9, randomly inserted, single clone1 550,00 €
Human cell line SNU-423 stably expressing Cas9, randomly inserted, single clone (indicated price is for Academic; Industrial customers please inquire)
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Human cell line SNU-387 stably expressing Cas9, randomly inserted, single clone1 550,00 €
Human cell line SNU-387 stably expressing Cas9, randomly inserted, single clone (indicated price is for Academic; Industrial customers please inquire)
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Human cell line RKO stably expressing Cas9, randomly inserted, single clone1 550,00 €
Human cell line RKO stably expressing Cas9, randomly inserted, single clone (indicated price is for Academic; Industrial customers please inquire)
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