ProteoSpin Endotoxin Removal Micro Kit

Supplier
Norgen Biotek
Cat. no.
1022800
Kit size
20
Links
Norgen Biotek Protocol Information Sheet

Features

• Endotoxin-free proteins and peptides - ProteoSpin™ Endotoxin Removal kits reduce endotoxin levels to less than 0.01 EU/µg of protein.
• Fast and easy processing - Efficient processing of multiple samples in 20 to 30 minutes using an easy-to-use protocol.
• High Recovery - Recovery is over 95% of input.
• Recovered protein is suitable for downstream applications - Purified protein is suitable for in vivo and in vitro introduction into cells and organisms, monoclonal and polyclonal antibody generation.

The ProteoSpin™ Endotoxin Removal Micro Kit is designed for the rapid removal of endotoxins from up to 150 µg of previously purified proteins or peptides. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications.

The ProteoSpin™ Endotoxin Removal Micro Kit efficiently reduces endotoxin levels to = 0.01 EU/µg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes. The ProteoSpin™ Endotoxin Removal Micro Kit contains sufficient materials for 20 purifications. Each column is able to remove endotoxins from up to 150 µg of previously purified proteins, with protein recoveries of > 95% being achieved. Preparation time for a single sample is approximately 20 minutes.

Maximum Protein Input	150 µg
Maximum Protein Volume Input	450 µL
Final Endotoxin Levels	< 0.01 EU/µg protein
Average Recovery	>95 %
Time to Complete 10 Purifications	20 minutes

Endotoxins were removed from 100 µg protein samples in triplicate using the ProteoSpin ™ Endotoxin Removal Micro Kit.

On average, the endotoxin levels in the 100 µg samples were reduced from 0.9 EU/ µg to 0.005 EU/ µg.

Average protein recoveries of greater then 95% were achieved when endotoxins were removed from 100 µg inputs of protein in triplicate.

User-Friendly Micro Kit Procedure

• in vivo and in vitro introduction into cells and organisms
• monoclonal and polyclonal antibody generation