Streptavidin Masterbeads 500nm 2ml

Supplier
Ademtech
Cat. No
2003151
Kit size
2 ml
Links
ademtech Protocol Information Sheet

Features

• Perfect spherical shape with an important surface area (5m2/g)
• High binding capacity: >800 pmol/mg
• 500nm MasterBeads Streptavidin: improved magnetic response and keep all benefits of Adembeads (no sedimentation, no aggregation); particularly suitable for automation.
• Unique size distribution: high reproducibility
• Many applications: immunoassays, protein purification, cell sorting or molecular biology purification (such as mRNA isolation)

Bio-Adembeads Streptavidin are monodispersed and superparamagnetic particles coated with natural streptavidin from S. avidinii. They are produced under asceptic conditions and are supplied at 1% (10mg/ml) and resuspended in Storage Buffer (#2010201),an aqueous suspension containing 0.05% Proclin 300. Streptavidin is a 60,000 dalton protein and composed of four identical subunits, each of which has a binding site for biotin. Bio-Adembeads Streptavidin are used for binding biotinylated ligands such as oligonucleotides, nucleic acids or proteins. Their high iron oxide content associated with the powerful streptavidin-biotin interaction, make Bio-Adembeads Streptavidin a very efficient tool for separation assays.

Simply wash the beads, resuspend in immobilization buffer (supplied with the beads)) and then immobilize the biotinylated molecule at room temp for 30 minutes.

Diameter	500 nm (CV max 20%)
Density	approx. 2.0g/cm3
Magnetisation at saturation	approx. 40emu/g
Specific surface area	5m2/g
Iron oxide content	approx. 70%
Solid content	10mg/ml

Bio-Adembeads Streptavidin are used for binding biotinylated ligands such as oligonucleotides, nucleic acids or proteins. Their high iron oxide content associated with the powerful streptavidin-biotin interaction, make Bio- Adembeads Streptavidin a very efficient tool for separation assays. Immunoassays, protein purification, cell sorting or molecular biology purification