Blue Cell Viability Assay Kit offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. The BLUE Cell viability kit detects cell viability by utilizing a blue indicator dye resazurin to measure the metabolic capacity of cells Learn More
Blue Cell Viability Assay Kit offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. The BLUE Cell viability kit detects cell viability by utilizing a blue indicator dye resazurin to measure the metabolic capacity of cells Learn More
Protein Carbonylation Assay Kit 100 tests (96 well plate)
Fast and easy method for the detection of Protein carbonylation in biological samples. Oxidative stress may cause reversible or irreversible changes in proteins. Such changes are meant to modulate protein function (redox regulation) or protect against irreversible damage that causes the inactive proteins to accumulate or become degraded.
The detection of carbonylated proteins is based on 2,4-dinitrophenylhydrazine (DNPH).
the reaction product (DNP) can be detected and quantified spectrophotometrycally at a typical absorption spectrum with a maximum at 365-375nm. Learn More
XcyteTM and XcyteTM Plus media supplements have been developed for the express purpose of providing xeno-free materials for the growth, expansion, and culture of human cells; stem cells in particular. When used as directed XcyteTM medias provide the complex mixture of growth factors, hormones, attachment factors, electrolytes and lipids needed to sustain healthy cells. Learn More
XcyteTM and XcyteTM Plus media supplements have been developed for the express purpose of providing xeno-free materials for the growth, expansion, and culture of human cells; stem cells in particular. When used as directed XcyteTM medias provide the complex mixture of growth factors, hormones, attachment factors, electrolytes and lipids needed to sustain healthy cells. Learn More
Nitrite / Nitrate Determination 100 tests (96 well plate)
Fast and easy method for the detection of nitric oxide formation in biological samples. This method relies on an innovative cofactor technology allowing linearity over a broad range of concentration (over 100 µM) and reducing the handling and assay time
The kit is suitable for different sample types: plasma, serum, saliva, urine, cell lysate, tissue homogenate and culture medium.
In this assay, firstly, the nitrate is reduced to nitrite catalyzed by the nitrate reductase with cofactors and specific compounds to eliminate interferences.
The detection is based on the final product detection (diazonium compound, λmáx=540nm) obtained after nitrite reaction in several steps with sulfanilamide. Learn More
Thiol Quantification Assay KIT (-SH/S-S) 200 tests (96 well plate)
The Thiol and Disulfide Assay Kit is recommended for estimations of oxidative stress levels in biological samples such as plasma. The method uses the measurement of oxidized and reduced thiols, using Ellman's reagent. This assay allows to determine both the native (naturally reduced) and total (chemically reduced) free thiols in each sample.
The the read-out is made at 412 nm (OD)
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ROS indicator, Ex/Em = 495/529 nm. H2DCFDA [2′,7′-dichlorodihydrofluorescein diacetate] is a chemically reduced analogue of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent H2DCFDA is converted to the highly fluorescent 2',7'-dichlorofluorescein.
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