AAV viral particles

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GeneCopoeia         PackGene
Product
AAV Adeno-associated viral particles
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PackGene PAckGene service information

Features

AAVPrime™ Adeno-associated virus (AAV) products are the ideal tools for inserting genes into a broad range of cell types with high efficiency and enhanced safety.  The optimized helper-free human AAV system allows viral packaging without potentially pathogenic helper adenovirus. Many pre-made particle options are available in 14 different serotypes, including fluorescent reporters (Figure 1), or you can have us produce custom AAV particles for you with genes up to 3 kb in length. Particle preparations are available in either standard (for in vitro use only; Figure 1) or purified (suitable for in vivo use; Figure 2) formats. 

Advantages

• High Titers: up to 10^14 GC/ml, total Genome Copies from 5x10^12 GC up to 10^15 GC

• Verstile: usable in a broad range of host cell types.

• Low toxicity: does not integrate into the host genome

• Low immunogenicity: minimal host immune response

 

• Safe: not associated with any human disease

 

AAVPrime™ standard AAV particles expressing fluorecent reporters:

 

Figure 1. HT1080 cells in 24-well plates were transduced with 0.5 µL of AAV standard  format particles expressing either eGFP (A), RFP (B), or mCherry (C)). Cells were visualized with a fluorescence microscope (Exposure time: 400 ms).

AAVPrime™ purified AAV particles expressing eGFP:

 

Figure 2. HT1080 cells in 24-well plates were transducted with different amounts (from left to right: 0.005 µl, 0.05 µl and 0.5 µl) of AAV purified particles expressing eGFP. Cells were visualized with a fluorescence microscope (Exposure time: 400 ms).
Different serotypes differ mainly in surface protein (capsid). Carefully choosing the serotype enables robust gene expression in specific tissues with minimal immune response. Refer to the "Serotypes & Vectors" to learn the essential differences between the different serotypes..

AAV serotypes available (GeneCopoeia) :

Vector choices (GeneCopoeia):

AAV vector collection enables efficient gene expression in a broad range of cell types. For insert lengths up to 3kb.
 
AAV shRNA



 

AAV serotypes available (PackGene) :

AAV1 to AAV10, AAV DJ/8, AAV rh10, AAV PHP.eB, PHP.S, 2-retro

Vector choices (PackGene):

up to 22 promoters available for gene overexpression:

Custom shRNA/ miRNA: M serial

Custom sgRNA (CRISPR spCas9, SaCas9/ miRNA: A/B/C serial

Premade AAV particles can be ordered from the list below

Scroll down this page for premade AAV particles list

Custom AAV particles production services are currently available for ORF expression:

We can deliver AAV particles with titers starting from 10^11 GC/mL or higher depending upon the request. Please follow the procedure hereunder:

1. Identify the gene of interest:

Please gather as much as possible of the following information: Gene Description, species, Nucleotide Accession, Entrez Gene ID, Symbols/Aliases or Catalog/product ID. You can also submit a sequence so we can search by target gene sequences homology (BLAST)

2. Choose the vector(s) of interest:

Pick-up the vector/expression system(s) you wish to use from the list (see "Vector choices")

3. Send us these information

We will contact you shortly to confirm: sequences, vectors and controls