PCR

Polymerase chain reaction (PCR)

The polymerase chain reaction (PCR) is a technique to amplify a short sequence of DNA even in samples containing only minute quantities of DNA. PCR is used to amplify selected sections of DNA or RNA across several orders of magnitude.

1. Top DNA polymerase is a novel thermostable DNA polymerase that is more processive than Taq DNA polymerase. In fact, the extension rate of Top DNA Polymerase is > 3 x that of Taq DNA Polymerase! Top DNA Polymerase can be used for a variety of PCR applications (including TA cloning) and is a robust enzyme for everyday PCR. It contains no proofreading or 5’-3’ Exonuclease activity. Also the use of this enzymed does not require any licence.                                                                                                                                                                                                                                                                                                                                                                                                                                                            

1. HotStart DNA polymerase uses an exclusive enzyme-mediated HotStart PCR method that, unlike most other HotStart PCR chemistries, completely releases all polymerase activity during the first denaturation step. Top DNA polymerase is completely inhibited by pyrophosphate at temperatures below 70°C. However, at temperatures above 70°C, a thermostable pyrophosphatase initiates pyrophosphate hydrolysis and activates the DNA polymerase. This prevents the formation of non-specific products and primer-dimers during the reaction set-up process and results in improved PCR specificity.  Also the use of this enzymed does not require any licence.                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        

1. HotStart Taq DNA Polymerase is designed to increase specificity and sensitivity in PCR. The HotStart Taq DNA polymerase is inhibited at temperatures lower than 70°C, but is fully activated after the first denaturation step. This prevents the formation of mis-primed products and primer-dimers during the reaction setup process, resulting in improved PCR specificity.                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      

1. ProFi Taq DNA polymerase, developed by Bioneer, is a unique recombinant Taq DNA polymerase that offers enhanced amplification efficiency for PCR. ProFi Taq DNA polymerase provides more efficient amplification and higher fidelity than conventional Taq DNA polymerase. This enzyme is applicable to any template DNA, and especially effective in amplifying large genomic DNA fragments up to 20 kb. ProFi Taq DNA polymerase provides accurate long-range amplification of standard and complex templates and amplification of low-copy target, and is highly suitable for all PCR applications.                                                                                                                                                                                                                         

1. Pfu DNA polymerase is a thermostable DNA polymerase isolated from Pyrococcus furiosus Vc1. It catalyzes the DNA-dependent polymerization of nucleotides into duplex DNA in the 5’ → 3’ direction and exhibits 3’ → 5’ exonuclease (proof reading) activity.  Pfu DNA polymerase is the ideal choice for a variety of techniques requiring high-fidelity DNA synthesis by PCR reaction.  It can apply to cloning, gene expression, site-directed mutagenesis and etc.                                                                                                                                                                                                                                                                                                                                                                                                                                                          
   

1. Taq DNA Polymerase is a thermostable DNA polymerase that catalyzes the polymerization of nucleotides into duplex DNA in the 5' -> 3' direction. Bioneer's Taq DNA Polymerase is isolated from recombinant E.coli strain containing the DNA polymerase gene from Thermus aquaticus YT1. It exhibits its highest activity at pH 9.0 and 72°C.