Lentiviral Packaging Systems
Our HIV and FIV Expression Packaging Systems includes an optimized lentiviral packaging plasmid mix, an eGFP positive control plasmid, a new transfection reagent, EndoFectin™ optimized for virus production and TiterBoost™ reagent that further increases the titers 5-10 fold. When combined with our ORF cDNA, shRNA or miRNA lentiviral constructs the results are high titers and robust expression levels.
The packaging plasmids in both HIV and FIV packaging systems are designed for coordinated, high-level expression of all essential lentiviral packaging elements to ensure efficient packaging of the recombinant transcript into infectious virus particles, as well as for preventing the viral sequences from self-replicating
Our 293Ta packaging cells for high titer lentivirus production using our packaging kits. Lenti-Pac 293Ta packaging cell line enables easy and efficient transfection for high-level expression of viral proteins.
We also offer a Lentivirus Concentration Solution is designed for quick and simple concentration of lentiviral particles. The concentration is accomplished by mixing the proprietary reagent with the lentiviral supernatant, followed by a short incubation and centrifugation with an ordinary centrifuge.