Adenovirus products are the ideal tools for expressing or modifying genes for in vitro and in vivo research. Adenovirus is a popular choice for gene delivery applications primarily because of its large packaging capacity, high transgene expression, and ability to infect most cell types; it is also widely used in cardiovascular, liver, muscle, lung, tumor, stem cell and other fields. Once inside the cell, recombinant adenovirus remains episomal and does not integrate into the host genome.
- High titers. Titer of recombinant adenovirus particles can be up to 1012 VP/ml.
- Versatile. Usable in a broad range of host cell and animals.
- Large packaging capacity. The packaging capacity of recombinant adenovirus can be up to 5 kb.
- High transduction efficiency.
- Low toxicity. Does not integrate into the host genome.
- Safe. Recombinant adenovirus is replication-incompetent (El (early protein) and/or E3 deletion mutants).
Figure 1. Hela, A549, and Vero cells in 12-well plates were transduced with the indicated amounts of eGFP ultra-purified AdenoFect™ Adenovirus particles (Cat#. AD05-EGFP-EB001, 3.39×1012vp/mL, 1.56 μl/well) at MOI 10. The expression of eGFP was visualized with a fluorescence microscope at 15, 24, and 48 hours post-transduction respectively.
Custom adenovirus production services are available for human, mouse and rat ORF cDNAs. For custom adenovirus packaging service, please contact us at firstname.lastname@example.org to get a quote.
Choose from our collection of adenovirus vectors with different promoters and fluorescent markers for your gene expression.