Exosome Quantification

Exosome capture and quantification using the ExoTEST™ Ready to Use Kit ensures dose-dependent exosome detection in a variety of samples. The quantification can be both relative and absolute as the assay calibration enables the calculation of the approximate exosome quantity for each sample.

Comprehensive exosome profiling is possible by implementing different combinations of capture and detection antibodies. ExoTEST™ can be customized for assessing multiple antigens in a total exosome population within a sample. Informative protein profiles for the samples of interest can be combined with subsequent nucleic acid (mRNA or miRNA) extraction and downstream analysis from captured exosomes.

Specific diagnostic applications using the ExoTEST™ allows the quantitative assessment of total circulating exosomes and specific exosomal sub-populations, defined by differentially expressed antigens under normal and pathological conditions.

Together with ExoTEST, HBM team has developed a new kit for FACS (Fluorescence Activated Cell Sorting) analysis of exosome markers, Exo-FACS, which allows exosome FACS profiling without wasting time in exosome isolation by ultracentrifugation.

Quantify exosomes in unfractionated samples easily in 3 steps:

• Exosome binding

• Antibodies binding

• Signal detection (either colorimetric, luminometric or fluorimetric)