Size Exclusion Chromatography
PURE-EVs:Size exclusion chromatography columns
Size Exclusion Chromatography (SEC) is considered one of the best methods for isolating and purifying exosomes and extracellular vesicles (EVs) from different matrices. In particular, this technique is very efficient for separating exosomes from the circulating proteins and does not affect the original shape and functionality of the vesicles. HBM has developed different classes of SEC columns for EV purification: PURE-EVs and miniPURE-EVs are two different size of columns for EV purification from small volume (2 ml up to 100 μl) of different matrices. In addition, HBM provides PURE-EVs PLUS, which, together with the SEC column, includes MWCO (molecular weight cut-off) concentrators especially designed for concentrating the EV preparation. Using PURE-EVs PLUS you can get in approximately 15 minutes a concentrate EV preparation.
Applications: Exosome isolation from biofluids and cell media. Purification of pre-isolated EVs from contaminants Multiple analysis of isolated exosomes (NTA, ELISA, FACS, WB, EM, MS, nucleic acid extraction, etc).
Advantages: Easy and fast protocol (turnaround time approximately 15 minutes). Isolate exosome from small volumes of sample. PURE-EVs PLUS includes MWCO concentrators especially designed for EV concentration. Reusable up to 5 times. Easy to store and ship (4°C).
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PURE-EVs: Size Exclusion Chromatography columns (5 columns)379,00 €PURE-EVs: Size exclusion chromatography columns (5 columns) for exosomes purification from biofluids or cell culture media Learn More
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PURE-EVs: Size Exclusion Chromatography columns (10 columns)689,00 €PURE-EVs: Size exclusion chromatography columns (10 columns) for exosomes purification from biofluids or cell culture media Learn More
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miniPURE-EVs: Size Exclusion Chromatography mini-columns (10 columns)350,00 €miniPURE-EVs: Size Exclusion Chromatography mini-columns (10 columns) for exosomes purification from biofluids or cell culture media Learn More
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miniPURE-EVs: Size Exclusion Chromatography mini-columns (20 columns)630,00 €miniPURE-EVs: Size Exclusion Chromatography mini-columns (20 columns) for exosomes purification from biofluids or cell culture media Learn More
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TFF-Easy filter cartridge220,00 €TFF-Easy filter cartridge in hollow fibers made of polysulfone, allowing concentration and removal of small proteins and molecules from diluted matrices (cell conditioned media, urine, etc.), prior to the EV purification. Learn More
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TFF-Easy filter cartridge (5 units)999,00 €
TFF-Easy filter cartridge in hollow fibers made of polysulfone, allowing concentration and removal of small proteins and molecules from diluted matrices (cell conditioned media, urine, etc.), prior to the EV purification. (set of 5 cartridges)
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TFF-EVs-Small- tangential flow filtration-based device230,00 €
TFF-EVs-Small - tangential flow filtration-based device
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TFF-EVs-Large- tangential flow filtration-based device390,00 €
TFF-EVs-Large- tangential flow filtration-based device
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TFF-EVs-MV- tangential flow filtration-based device220,00 €
TFF-EVs-MV- tangential flow filtration-based device
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