OmicsLink™ shRNA clone collections include lentiviral and non-viral vector-based shRNA constructs against genome-wide human, mouse and rat genes. shRNA of varying lengths (19 to 29 bases) were designed using a proprietary algorithm to make shRNA expression constructs that have high knockdown efficiency with minimal off-target effect.
Guaranteed shRNA knockdown
A set of four expression constructs is offered against every target gene with the guarantee that at least one of the four will have a knockdown effect of 70% or more on corresponding gene expression as determined by qRT-PCR, otherwise the constructs will be replaced one time free of charge
All cell types covered
Lentiviral and non-viral vector option allows transfection or transduction of shRNA into difficult-to-transfect cells as well conventional dividing cell lines.
Multiple delivery formats
shRNA are delivered as 4 individual constructs of 5 µg purified plasmid. Lentivirus for shRNA is also available as a part of custom services
Markers and reporters
Vectors with mCherry or eGFP reporter gene for monitoring transfection or transduction efficiencies. Stable cell selection with puromycin marker.
Fully sequenced expression cassettes
Expression cassettes of all shRNA clones are fully sequenced including the promoter, sense and antisense target sequences, hairpin, termination and other linker sequences.
Vector based shRNA technology. Collection of validated human kinome shRNA expression clones available in both regular mammalian expression vector psi-H1™ and lentiviral expression systems (psiLv-H1™ and psiLV-U6™), which enables both transient and stable Learn More
Vector based shRNA technology. Collection of shRNA expression clones available in both regular mammalian expression vector psi-H1™ and lentiviral expression systems (psiLv-H1™ and psiLV-U6™), which enables both transient and stable gene silencing. A mamma Learn More