While ZFNs were the first Engineered Nucleases of notable interest for gene targeting , they are largely surpassed now by TALENs and CRISPRs (RGENs) in terms of specificity and efficiency. We can nevertheless still provide with ZFNs if requested.
Zinc finger nucleases (ZFNs) are artificial restriction enzymes, created by fusing an engineered zinc finger DNA binding domain
(which targets a desired sequences) with the DNA cleavage domain of FokI, a type II restriction enzyme. Learn More