Cell Culture & Cell based assays

GeneCellin is a powerful in vitro transfection reagent which allows to achieve higher delivery efficiencies of plasmid DNA into cell lines and primary cells compare to other lipid and polymer based transfection reagents. This innovative transfection reage Learn More

Nitrite Determination 1000 tests (96 well plate) The Nitrite Determination Assay Kit is recommended for the determination of nitrite. The assay described here measures the nitrite anion. The detection is based of spectrophotometric properties of diazonium compound (λmax= 540 nm) obtained after several steps of nitrite reaction with sulfanilamide. Learn More

Protein Carbonylation Assay Kit 100 tests (96 well plate) Fast and easy method for the detection of Protein carbonylation in biological samples. Oxidative stress may cause reversible or irreversible changes in proteins. Such changes are meant to modulate protein function (redox regulation) or protect against irreversible damage that causes the inactive proteins to accumulate or become degraded. The detection of carbonylated proteins is based on 2,4-dinitrophenylhydrazine (DNPH). the reaction product (DNP) can be detected and quantified spectrophotometrycally at a typical absorption spectrum with a maximum at 365-375nm. Learn More

Lipid Hydroperoxide Assay Kit (LPO) 200 tests (96 well plate) Fast and easy method for the detection of lipid peroxidation in biological samples. The LPO assay kit measures MDA and HNE concentrations as an index of lipid peroxidation The kit is suitable for different sample types: plasma, tissue homogenate and cultured cells. Learn More

Thiol Quantification Assay KIT (-SH/S-S) 200 tests (96 well plate) The Thiol and Disulfide Assay Kit is recommended for estimations of oxidative stress levels in biological samples such as plasma. The method uses the measurement of oxidized and reduced thiols, using Ellman's reagent. This assay allows to determine both the native (naturally reduced) and total (chemically reduced) free thiols in each sample. The the read-out is made at 412 nm (OD) Learn More

Superoxide indicator, Ex/Em = 300/610 nm. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (Ex/Em: 355/420nm) in cells, while the oxidized form ethidium has red fluorescence (Ex/Em: 300/610 nm) upon DNA intercalation. Learn More

ROS indicator, Ex/Em = 507/529 nm. Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. Dihydrorhodamine 123 itself is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide and peroxynitrite. Learn More

ROS indicator, Ex/Em = 528/551 nm. Dihydrorhodamine 6G is the reduced form of rhodamine 6G, which is used as fluorescent mitochondrial dye. It is nonfluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 6G that accumulates in mitochondrial membranes. Dihydrorhodamine 6G is useful for detecting reactive oxygen species (ROS) including superoxide Learn More

ROS indicator, Ex/Em = 495/529 nm. H2DCFDA [2′,7′-dichlorodihydrofluorescein diacetate] is a chemically reduced analogue of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent H2DCFDA is converted to the highly fluorescent 2',7'-dichlorofluorescein. Learn More

ROS indicator, Ex/Em = 495/529 nm. Carboxy-H2DCFDA [5-(and 6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate] is a chemically reduced analogue of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent Carboxy-H2DCFDA is converted to the highly fluorescent 5-(and 6)-carboxy-2',7'-dichlorofluorescein, with additional negative charges that impede its leakage out of the cell. Learn More