Cell Culture & Cell based assays

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  1. Nitrite / Nitrate Determination  100 tests
    Nitrite / Nitrate Determination 100 tests
    235,00 €
    Nitrite / Nitrate Determination 100 tests (96 well plate) Fast and easy method for the detection of nitric oxide formation in biological samples. This method relies on an innovative cofactor technology allowing linearity over a broad range of concentration (over 100 µM) and reducing the handling and assay time The kit is suitable for different sample types: plasma, serum, saliva, urine, cell lysate, tissue homogenate and culture medium. In this assay, firstly, the nitrate is reduced to nitrite catalyzed by the nitrate reductase with cofactors and specific compounds to eliminate interferences. The detection is based on the final product detection (diazonium compound, λmáx=540nm) obtained after nitrite reaction in several steps with sulfanilamide. Learn More
  2. Protein Carbonylation Assay Kit
    Protein Carbonylation Assay Kit
    330,00 €
    Protein Carbonylation Assay Kit 100 tests (96 well plate) Fast and easy method for the detection of Protein carbonylation in biological samples. Oxidative stress may cause reversible or irreversible changes in proteins. Such changes are meant to modulate protein function (redox regulation) or protect against irreversible damage that causes the inactive proteins to accumulate or become degraded. The detection of carbonylated proteins is based on 2,4-dinitrophenylhydrazine (DNPH). the reaction product (DNP) can be detected and quantified spectrophotometrycally at a typical absorption spectrum with a maximum at 365-375nm. Learn More
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