Cell Culture & Cell based assays

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  1. Protein Carbonylation Assay Kit
    Protein Carbonylation Assay Kit
    330,00 €
    Protein Carbonylation Assay Kit 100 tests (96 well plate) Fast and easy method for the detection of Protein carbonylation in biological samples. Oxidative stress may cause reversible or irreversible changes in proteins. Such changes are meant to modulate protein function (redox regulation) or protect against irreversible damage that causes the inactive proteins to accumulate or become degraded. The detection of carbonylated proteins is based on 2,4-dinitrophenylhydrazine (DNPH). the reaction product (DNP) can be detected and quantified spectrophotometrycally at a typical absorption spectrum with a maximum at 365-375nm. Learn More
  2. Thiol Quantification Assay KIT (-SH/S-S)  200 tests
    Thiol Quantification Assay KIT (-SH/S-S) 200 tests
    315,00 €
    Thiol Quantification Assay KIT (-SH/S-S) 200 tests (96 well plate) The Thiol and Disulfide Assay Kit is recommended for estimations of oxidative stress levels in biological samples such as plasma. The method uses the measurement of oxidized and reduced thiols, using Ellman's reagent. This assay allows to determine both the native (naturally reduced) and total (chemically reduced) free thiols in each sample. The the read-out is made at 412 nm (OD) Learn More
  3. DHE [Dihydroethidium]  Superoxide indicator
    DHE [Dihydroethidium] Superoxide indicator
    160,00 €
    Superoxide indicator, Ex/Em = 300/610 nm. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (Ex/Em: 355/420nm) in cells, while the oxidized form ethidium has red fluorescence (Ex/Em: 300/610 nm) upon DNA intercalation. Learn More
  4. DHR 123  ROS indicator
    DHR 123 ROS indicator
    160,00 €
    ROS indicator, Ex/Em = 507/529 nm. Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. Dihydrorhodamine 123 itself is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide and peroxynitrite. Learn More
  5. DHR 6G  ROS indicator
    DHR 6G ROS indicator
    160,00 €
    ROS indicator, Ex/Em = 528/551 nm. Dihydrorhodamine 6G is the reduced form of rhodamine 6G, which is used as fluorescent mitochondrial dye. It is nonfluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 6G that accumulates in mitochondrial membranes. Dihydrorhodamine 6G is useful for detecting reactive oxygen species (ROS) including superoxide Learn More
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