Nitrite / Nitrate Determination 100 tests (96 well plate)
Fast and easy method for the detection of nitric oxide formation in biological samples. This method relies on an innovative cofactor technology allowing linearity over a broad range of concentration (over 100 µM) and reducing the handling and assay time
The kit is suitable for different sample types: plasma, serum, saliva, urine, cell lysate, tissue homogenate and culture medium.
In this assay, firstly, the nitrate is reduced to nitrite catalyzed by the nitrate reductase with cofactors and specific compounds to eliminate interferences.
The detection is based on the final product detection (diazonium compound, λmáx=540nm) obtained after nitrite reaction in several steps with sulfanilamide. Learn More
Nitrite Determination 1000 tests (96 well plate)
The Nitrite Determination Assay Kit is recommended for the determination of nitrite.
The assay described here measures the nitrite anion. The detection is based of spectrophotometric properties of diazonium compound (λmax= 540 nm) obtained after several steps of nitrite reaction with sulfanilamide. Learn More
Protein Carbonylation Assay Kit 100 tests (96 well plate)
Fast and easy method for the detection of Protein carbonylation in biological samples. Oxidative stress may cause reversible or irreversible changes in proteins. Such changes are meant to modulate protein function (redox regulation) or protect against irreversible damage that causes the inactive proteins to accumulate or become degraded.
The detection of carbonylated proteins is based on 2,4-dinitrophenylhydrazine (DNPH).
the reaction product (DNP) can be detected and quantified spectrophotometrycally at a typical absorption spectrum with a maximum at 365-375nm. Learn More
Thiol Quantification Assay KIT (-SH/S-S) 200 tests (96 well plate)
The Thiol and Disulfide Assay Kit is recommended for estimations of oxidative stress levels in biological samples such as plasma. The method uses the measurement of oxidized and reduced thiols, using Ellman's reagent. This assay allows to determine both the native (naturally reduced) and total (chemically reduced) free thiols in each sample.
The the read-out is made at 412 nm (OD)
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Nitrite / Nitrate Determination 100 tests235,00 €Nitrite / Nitrate Determination 100 tests (96 well plate) Fast and easy method for the detection of nitric oxide formation in biological samples. This method relies on an innovative cofactor technology allowing linearity over a broad range of concentration (over 100 µM) and reducing the handling and assay time The kit is suitable for different sample types: plasma, serum, saliva, urine, cell lysate, tissue homogenate and culture medium. In this assay, firstly, the nitrate is reduced to nitrite catalyzed by the nitrate reductase with cofactors and specific compounds to eliminate interferences. The detection is based on the final product detection (diazonium compound, λmáx=540nm) obtained after nitrite reaction in several steps with sulfanilamide. Learn More
Nitrite Determination 1000 tests208,00 €Nitrite Determination 1000 tests (96 well plate) The Nitrite Determination Assay Kit is recommended for the determination of nitrite. The assay described here measures the nitrite anion. The detection is based of spectrophotometric properties of diazonium compound (λmax= 540 nm) obtained after several steps of nitrite reaction with sulfanilamide. Learn More
Protein Carbonylation Assay Kit330,00 €Protein Carbonylation Assay Kit 100 tests (96 well plate) Fast and easy method for the detection of Protein carbonylation in biological samples. Oxidative stress may cause reversible or irreversible changes in proteins. Such changes are meant to modulate protein function (redox regulation) or protect against irreversible damage that causes the inactive proteins to accumulate or become degraded. The detection of carbonylated proteins is based on 2,4-dinitrophenylhydrazine (DNPH). the reaction product (DNP) can be detected and quantified spectrophotometrycally at a typical absorption spectrum with a maximum at 365-375nm. Learn More
Thiol Quantification Assay KIT (-SH/S-S) 200 tests315,00 €Thiol Quantification Assay KIT (-SH/S-S) 200 tests (96 well plate) The Thiol and Disulfide Assay Kit is recommended for estimations of oxidative stress levels in biological samples such as plasma. The method uses the measurement of oxidized and reduced thiols, using Ellman's reagent. This assay allows to determine both the native (naturally reduced) and total (chemically reduced) free thiols in each sample. The the read-out is made at 412 nm (OD) Learn More