Expression clones

• MicroRNAs (miRNAs) are small, non-coding single stranded RNA molecules found in eukaryotic organisms. They are highly conserved and usually 21-23 nucleotides in length. miRNAs are involved in almost all cellular functions and regulate gene expression by binding to the 3' untranslated regions (3' UTRs) of targeted mRNAs. Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases.

• LabOmics offers miExpress precursor miRNA expression clones in lentiviral and non-viral vectors with eGFP reporter gene. Hairpin precursor miRNA of approximately 150 nucleotides is cloned into lentiviral or non-viral vectors for delivery in virtually all cell types.

Advantages

• Full coverage

• All known human, mouse and rat miRNAs in the current miRBase covered.

• Flexible delivery systems

• Lentiviral vectors allow efficient transduction and stable integration into the host genome of non-dividing and difficult to transfect cells target cells

  Non-viral vectors allow either transient or stable transfection.

• Optimized expression cassettes

• Expression cassettes have been fully sequenced and optimized for high miRNA expression of precursor miRNA and maturation of miRNA inside cells

• Selection markers and reporters

• An eGFP reporter is designed to monitor transfection or transduction efficiency

  Neomycin or Puromycin selection marker enables stable cell line selection

Precursor miRNA vector features

• Vector

• pEZX-MR01

• pEZX-MR02

• pEZX-MR03

• Tracking-gene

• eGFP

• eGFP

• eGFP

• Selection marker

• Neomycin

• Puromycin

• Puromycin

• Promoter

• H1

• CMV

• CMV

• Type

• FIV

• HIV

• Mammalian