RT PCR

Reverse Transcription (RT) and Reverse Transcription and amplification by PCR (RT-PCR)

1. AccuPower® CycleScript RT (available with: dT₂₀, dN₁₂, or dN₆ primers) PreMix is an easy to resuspend lyophilized PCR Master Mix of CycleScript Reverse Transcriptase, a primer, and all of the other components for cDNA synthesis conveniently packaged in individual tubes. Simply add template RNA, and water. Then, the reverse transcription is performed by either a Cyclic RT reaction (patent pending) or conventional reverse transcription PCR. The use of Cyclic RT produces cDNA amplification and better results compared to conventional reverse transcription PCR – especially for rare transcripts.                                                                                                                                                                                                                                                                                                                                                                                                                                                            

1. AccuPower® RT PreMix is a master mix for cDNA synthesis that consists of an easy to resuspend, lyophilized mix of M-MLV Reverse Transcriptase, RNase inhibitor, dNTPs, reaction buffer, tracking dye, and patented stabilizer. The master mix kit is used for first strand cDNA synthesis from RNA. Downstream applications include cDNA amplification with reverse transcription PCR. All of the key components are premixed at optimal concentrations. Simply add template RNA, primers and water to start your reaction.                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        

1. AccuPower® RT-PCR PreMix contains all the components necessary for sequential cDNA synthesis and amplification in one tube (one-step RT-PCR). This RT-PCR PreMix consists of both M-MLV Reverse Transcriptase, RNA dependent DNA polymerase, and a thermostable DNA polymerase in a lyophilized mix of dNTPs, reaction buffer, RNase inhibitor, tracking dye, and stabilizer. The kit can be used for double stranded cDNA synthesis from low copy RNA or mRNA and subsequent RT-PCR.                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      

1. AccuPower® RocketScript™ RT PreMix contains Bioneer’s exclusive M-MLV based thermostable reverse transcriptase (RTase), RocketScript™. Native M-MLV RTase has maximum activity at relatively low temperatures (42°C), causing several problems in reverse transcription of RNA molecules with complex secondary structure. RocketScript™ has thermostable activity (42°C~70°C), allowing efficient cDNA synthesis from virtually any RNA. The lyophilized PreMix contains all components necessary for a successful reverse transcription reaction, including RTase, RNase inhibitor and buffer components. Just add template RNA, primers and water, and the RT reaction is ready to go.                                                                                                                                                                                                                         

1. AccuPower^® RocketScript™ Cycle RT PreMix contains Bioneer’s exclusive M-MLV based thermostable reverse transcriptase (RTase), RocketScript. Conditions are optimized for Bioneer’s Cyclic Temperature Reverse Transcription (CTRT; patent pending) in a premix form.                                                                                                                                                                                                                                                                                                                                                                                                                                                          
   

1. AccuPower® RocketScript™ RT-PCR PreMix contains Bioneer’s exclusive M-MLV based thermostable reverse transcriptase (RTase) and Top DNA polymerase, dNTPs and reaction buffer components all in a single, easy to use, one-step RT-PCR product. By using RocketScript™ RTase, full-length cDNA are efficiently synthesized from complex secondary structure RNA species. RocketScript™ also has outstanding extension properties, allowing for highly-sensitive and high-yielding cDNA synthesis of low copy targets. The convenient lyophilized PreMix also contains tracking dye (blue and yellow) and also a density-increasing agent, allowing the user to directly place the reaction mixture in an agarose gel and perform electrophoresis.                                                                                                                                                                                                                 

3. AccuPower^® RocketScript™ RT PreMix, RNase H Minus is a ready-to-use lyophilized premix containing all components, including RocketScript™ Reverse Transcriptase, RNase H Minus, for first-strand cDNA synthesis from purified Poly(A) or total RNA template. RocketScript™ Reverse Transcriptase, RNase H Minus is a recombinant M-MLV reverse transcriptase with enhanced thermostability up to 70⁰C, as well as eliminated RNase H activity. It is highly efficient in producing full-length cDNA from long RNA transcripts up to 12.5 kb. AccuPower^® RocketScript™ RT PreMix, RNase H Minus is capable of producing cDNA from as little as 1 pg of total RNA for real-time RT-PCR (qRT-PCR) analysis and other applications.                                                                                                                                                                                                                                   
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5. AccuPower® Rocketplex RT-PCR PreMix is a new product that is in a class by itself. It provides you with the best, most thermostable Reverse transcription available, while giving you multiplex capabilities in the subsequent PCR. By combining Multiplex RT-PCR with RocketScript™ Reverse Transcriptase you can amplify from 1 to more than 10 target genes.AccuPower® Rocketplex RT-PCR PreMix contains all the components necessary for sequential cDNA synthesis and amplification of multiple targets in one tube (one-step RT-PCR). The RT-PCR PreMix consists of both RocketScript™ Reverse Transcriptase, an RNA dependent DNA polymerase, and a thermostable DNA polymerase in a lyophilized mix of dNTPs, reaction buffer, RNase inhibitor, Loading dye and stabilizer.                                                                                                                                                                                                                                                                                                                                                                                                                                           
7. AccuPower^® Dual-HotStart™ RT-PCR PreMix (PCT/KR2013001935, US14383793, EP13757504, etc.) is a breakthrough product to overcome frequent problems from RT reaction. By using this proprietary product, cDNA synthesis of your selective RNA will be performed with high sensitivity from a small amount of template RNA. Successive PCR amplification of synthesized cDNA also occurs via a one-step reaction with cDNA synthesis. This one-step RT-PCR product is easily generated with excellent sensitivity for a wide range of applications, including several types of virus tests and gene expression analysis experiments. This product is ready-to-use, simply add template RNA, primers and DEPC-DW, and obtain your results with great reproducibility.