5 x HOT FIREPol® Probe qPCR Mix Plus (ROX)

Supplier
LabOmics Solis Biodyne
Cat. No
6325000
Pack size
20ml (5000rxn)
Links
Data Sheet MSDS

Features

• optimized for real-time quantitative PCR assays
• enables detection of low copy number targets
• prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures

HOT FIREPol® Probe qPCR Mix Plus(ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

• ROX Dye: ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR. The high ROX concentration in the final 1x reaction is 500 nM.
• Shipping and Storage conditions: Routine storage: -20ºC Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® Probe qPCR Mix (high ROX).
• Mix Composition:

• HOT FIREPol® DNA Polymerase
• 5 x Probe qPCR buffer
• 15 mM MgCl2

1 x PCR solution – 3 mM MgCl2

• dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP
• 2.5 μM ROX dye

1 x PCR solution – 500 nM ROX dye

HOT FIREPol® Probe qPCR Mix Plus outperforms a leading qPCR Mix.

Serial of tenfold dilutions of 72 bp fragment of Albumin gene was amplified from human genomic DNA. Reactions were performed on an Applied Biosystems 7900HT Real-Time PCR System following cycling protocols recommended by the suppliers.

• Detection and quantification of DNA and cDNA targets
• Profiling gene expression
• Microbial detection
• Viral load determination