5x HOT FIREPol EvaGreen® qPCR Mix Plus (no ROX)
optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye (same usage but higher performances than Sybr green)
£72 / €74
| Supplier |
|---|
| LabOmics Solis Biodyne |
| Cat. No |
| 6340250 |
| Pack size |
| 1ml (250rxn) |
| Links |
Data Sheet MSDS |
Features
- much less PCR inhibition compare to SYBR® Green
- extremely stable dye
- nonmutagenic and noncytotoxic
HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers. HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
- EvaGreen® Dye: EvaGreen® is a DNA-binding dye with many features that make it a superior alternative to SYBR® Green I for qPCR. Apart from having similar spectra, EvaGreen® has three important features that set it apart from SYBR® Green I: EvaGreen® has much less PCR inhibition, is extremely stable dye and has been shown to be nonmutagenic and noncytotoxic. EvaGreen® is compatible with all common real-time PCR cyclers – simply select the standard settings for SYBR® Green or FAM!
- Shipping and Storage conditions: Routine storage: -20ºC Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® qPCR Mix Plus(no ROX).
- Mix Composition:
1 x PCR solution – 2.5 mM MgCl2
- HOT FIREPol® DNA Polymerase
- 5 x EvaGreen® qPCR buffer
- 12.5 mM MgCl2
- dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP
- EvaGreen® dye
- No ROX dye
Log scale amplification plot of FBA1 gene.
Amplification of a 92 bp fragment from FBA1 gene was performed on serial fourfold dilutions of Saccharomyces cerevisiae genomic DNA. Reactions were performed on an Applied Biosystems 7900HT Real-Time PCR System and ROX was used as a passive reference for normalisation of the reporter signal.
- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination
- High Resolution Melt (HRM) Analysis
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