96 mRNA Isolation Kit

In stock
SKU
2006031
295,00 €
96 mRNA Isolation Kit
£270 / €295

Supplier
Ademtech
Cat. No
2006031
Kit size
96 preps
Links
ademtech
Protocol
Information Sheet

Features

  • Complete kit adapted for Total RNA, Cells or whole blood
  • Microplate format: convenient for many mRNA isolation
  • More convenient for many subsequent RT-PCR
  • Very low ribosomal contamination
  • Intact and pure mRNA without degradation
  • Purification of all mRNA sizes
  • Fast system: less than 30 minutes

mRNA purification procedure relies on the base pairing between the poly A tail of the mRNA and the biotinylated oligonucleotides (dT)25 sequence bound to the Bio-Adembeads Streptavidin. Nucleo-Adembeads consists of superparamagnetic nanoparticles of uniform size and a perfect spherical shape. Oligonucleotides (dT)25 are linked to the surface of the superparamagnetic nanoparticles via Biotin-Streptavidin system. The true spherical shape eliminates n-specific binding associates with irregulary shape particles. After processing the protocol using Nucleo-Adembeads, the mRNA is ready to use for downstream applications such as RT-PCR, Nothern Blotting, cDNA library construction, nuclease protection assay, in vitro translation, primer extension, subtractive cDNA cloning and reverse transcription.
  • Prepare samples adequately with protocol (Total RNA, Cell lysate or whole blood)
  • Add prepared samples solutions to the [Oligo (dT)25 - Bio-Adembeads Streptavidin] complex and mix by pipetting.
  • Incubate for 10 minutes at room temperature.
  • Place the plate on the magnet, remove and discard the supernatants. Move off the plate and wash with: - 100 μl Washing Buffer for mRNA purification starting from Total RNA. Repeat twice this step. - 150 μl Lysis Buffer for mRNA purification starting from cells or whole blood.Repeat twice this step using 200μl of Washing Buffer.
  • Place the plate on the magnet (also available from Labomics), remove and discard the supernatants.
  • Move off the plate and resuspend the complexes in desired volume of Nuclease free Water (mix by pipetting).
  • After 2 minutes at room temperature, place the plate on the magnet during 5 minutes and transfer the supernatants (containing the mRNA) into a collecting plate for storage.
Beads 1 mL Bio-Adembeads Streptavidin
Buffers 30 mL Lysis Buffer 15 mL Binding Buffer 3X 40 mL Washing Buffer
Other 15 mL Nuclease free Water Lyophilisate Biotinylated Oligo(dT)25
Storage Store at 2-8°C.


  • mRNA purification from up to 25 µg Total RNA, 105 cells from Cell lysate or 25 µL whole blood
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