AccuTarget Luciferase siRNA

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AccuTarget Positive control Luciferase siRNA
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Features

• Controls offered at 10, 20, 50 and 100 nmole.



• AccuTargetTM Positive siRNAs can induce effective knockdown of target genes. As an endogenous gene, we offer a positive control siRNA targeting GAPDH and as a reporter system, positive control siRNAs targeting GFP and luciferase genes are available.

Hela cells in a 6-well plate were cotransfected with 400 ng of CMV-luc plasmid and 10 nM of luciferase siRNA using lipofectamine 2000 transfection reagent. Next day, cells were harvested and assayed for luciferase activity. As shown in Fig. 14, cotransfection with our positive control luciferase siRNA led to efficient knockdown of luciferase activity (85% - 95% knockdown compared to luciferase activity of NC-siRNA-transfected cells).

Control siRNA, BioRP purification	1 nmole: 60 euros	5 nmole: 85 euros	10 nmole: 160 euros	20 nmole: 195 euros	50 nmole: 295 euros
Control siRNA, HPLC purification	1 nmole: 90 euros	5 nmole: 160 euros	10 nmole: 210 euros	20 nmole: 295 euros	50 nmole: 370 euros
Control set (5 nmole (+), 2 nmole(-), BioRP purification	160 euros
Control set (5 nmole (+), 2 nmole(-), HPLC purification	198 euros
Fluorescein Labeled Negative, HPLC purification	1 nmole: 98 euros	5 nmole: 188 euros	10 nmole: 280 euros	20 nmole: 365 euros	50 nmole: 440 euros