Bio-Adembeads Protein A (2 ml)

Supplier
Ademtech
Cat. No
2004230
Kit size
2 ml
Links
ademtech Protocol Information Sheet

Features

• Convenient for small scale Ig purification and immunoprecipitation
• perfect spherical shape with an important smooth surface area (10 square meter/g)
• Starting sample can be saliva, plasma, ascites, serum and tissue culture or hybridoma supernatants.
• Recombinant form of the protein A without albumin-binding sites: reduces co-purification of contaminating proteins. Only the Fc binding sites are present.
• Magnetic technology: high specificity and convenience (no centrifugation)
• Rapid procedure:
• Convenient for a large number of immunoprecipitation: up to 100 per ml

Bio-Adembeads (300nm) coated with recombinant high quality Protein A covalently coupled to the surface. Protein A is a cell wall component composed of a single polypeptide chain of molecular weight 42 kDa produced by Staphylococcous aereus (Cowan Strain). The protein A employed  is recombinant form containing the Fc binding sites without any albumin binding sites. Protein A is capable of binding to the Fc portion of immunoglobulins, especially IgGs, from a large number of species. Protein A will bind the Fc portion of human IgG subclasses, IgM, IgA and IgE; and mouse IgG1 (weakly), IgG2a and IgG2b. Protein A also binds IgGs from other species, including monkey, rabbit, pig, guinea pig, dog and cat.

Immunoprecipitation Cells are lysed and cell debris are removed. A specific antibody is first added to the cell lysate. The Bio-Adembeads Protein A are then incubated with the cell lysate in order to form a magnetic labelled immune complex wich can be easily separated from the liquid by removing the supernatant during the magnetization step. Immunoglobulin purification Bio-Adembeads Protein A are particularly suitable for immunoglobulin purification from ascite, serum, tissue culture supernatants or other samples. After a short incubation time the immunoglobulins are eluted from the Bio-Adembeads Protein A and the containing Ig supernatant is pipetted off for downstream applications.

Diameter	300 nm (CV max 20%)
Magnetic susceptibility	approx. 40 emu/g
Specific surface area	10 m2/g
Iron oxide content	approx. 70%
Binding capacity	~100µg/ml
Number of reactions	100 immunoprecipitations (Cat No 2004230) / 250 immunoprecipitations (Cat No 2004231)
Storage	Supplied in storage buffer (Cat No 1010201) proclin 300 as a preservative Store at 2-8°C. The Bio-Adembeads Protein A must be maintained in liquid during storage and all handling steps. Drying will result in reduced performance. Do not freeze the product.

• Immunoprecipitation
• Co-immunoprecipitation
• Immunoglobulin purification