Bio-Adembeads Protein A/G (2 ml)

Supplier
Ademtech
Cat. No
2004630
Kit size
2 ml
Links
ademtech Protocol Information Sheet

Features

• High specificity-No background non-porous spherical beads, smooth surface - Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. - Best for IgG from many species; including all IgG that bind to PA and PG. Ideal choice for polyclonal or monoclonal IgG
• Simplifies and speeds IP,Co-IP & Immunoglobulin purification procedures
• No preclearing step required
• Less incubation time
• Washing step are reduced
• Optional cross-linking procedure included
• No centrifugation
• Clear visualization of the magnetic particles
• Gentle system Minimal loss of protein: smaller beads provide larger surface area and will hence give higher yields of protein - Minimal stress versus centrifugation - Isolation of large protein complex - Flexible: binds to a wider range of antibodies - Recombinant form of the protein AG without albumin-binding sites, thus reducing co-purification of contaminating proteins. Only the Fc binding sites are present - PAG Elution Buffer: optimized elution method

Bio-Adembeads (300nm) coated with recombinant high quality Protein AG covalently coupled to the surface. Protein AG is a genetically-engineered protein that combines the IgG binding domains of both Protein A and G. Recombinant fusion protein AG contains five Ig-binding regions of protein A and three Ig-binding. Protein AG binds to all IgG subclasses from various mammalian species, including all IgGs that bind both Protein A and Protein G, making the ideal choice for purification of all kinds of polyclonal and monoclonal IgG antibodies. The protein AG lacks albumin, cell wall binding regions and others non-specific binding regions to ensure maximum specific IgG binding.

Immunoprecipitation Cells are lysed and cell debris are removed. A specific antibody is first added to the cell lysate. The Bio-Adembeads PAG are then incubated with the cell lysate in order to form a magnetic labelled immune complex wich can be easily separated from the liquid by removing the supernatant during the magnetization step. Immunoglobulin purification Bio-Adembeads PAG are particularly suitable for immunoglobulin purification from ascite, serum, tissue culture supernatants or other samples. After a short incubation time the immunoglobulins are eluted from the Bio-Adembeads PAG and the containing Ig supernatant is pipetted off for downstream applications.

Diameter	300 nm (CV max 20%)
Magnetic susceptibility	approx. 40 emu/g
Specific surface area	10 m2/g
Iron oxide content	approx. 70%
Binding capacity	~100µg/ml
Number of reactions	100 immunoprecipitations (Cat No 2004630) / 250 immunoprecipitations (Cat No 2004631)
Storage	Supplied in storage buffer (Cat No 1010201) proclin 300 as a preservative Store at 2-8°C. The Bio-Adembeads PAG must be maintained in liquid during storage and all handling steps. Drying will result in reduced performance. Do not freeze the product.

• Immunoprecipitation
• Co-immunoprecipitation
• Immunoglobulin purification