Bio-Adembeads Protein A/G (5 ml)

In stock
SKU
2004631
640,00 €
Magnetic beads coated with a recombinant fusion protein A and G. Simplifies and speeds IP,Co-IP & Immunoglobulin purification procedures. High specificity-No background non-porous spherical beads, smooth surface - Cell wall binding region, albumin binding
£590 / €640

Supplier
Ademtech
Cat. No
2004631
Kit size
5 ml
Links
ademtech
Protocol
Information Sheet

Features

  • High specificity-No background non-porous spherical beads, smooth surface - Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. - Best for IgG from many species; including all IgG that bind to PA and PG. Ideal choice for polyclonal or monoclonal IgG
  • Simplifies and speeds IP,Co-IP & Immunoglobulin purification procedures
  • No preclearing step required
  • Less incubation time
  • Washing step are reduced
  • Optional cross-linking procedure included
  • No centrifugation
  • Clear visualization of the magnetic particles
  • Gentle system Minimal loss of protein: smaller beads provide larger surface area and will hence give higher yields of protein - Minimal stress versus centrifugation - Isolation of large protein complex - Flexible: binds to a wider range of antibodies - Recombinant form of the protein AG without albumin-binding sites, thus reducing co-purification of contaminating proteins. Only the Fc binding sites are present - PAG Elution Buffer: optimized elution method

Bio-Adembeads (300nm) coated with recombinant high quality Protein AG covalently coupled to the surface. Protein AG is a genetically-engineered protein that combines the IgG binding domains of both Protein A and G. Recombinant fusion protein AG contains five Ig-binding regions of protein A and three Ig-binding. Protein AG binds to all IgG subclasses from various mammalian species, including all IgGs that bind both Protein A and Protein G, making the ideal choice for purification of all kinds of polyclonal and monoclonal IgG antibodies. The protein AG lacks albumin, cell wall binding regions and others non-specific binding regions to ensure maximum specific IgG binding.
Immunoprecipitation Cells are lysed and cell debris are removed. A specific antibody is first added to the cell lysate. The Bio-Adembeads PAG are then incubated with the cell lysate in order to form a magnetic labelled immune complex wich can be easily separated from the liquid by removing the supernatant during the magnetization step. Immunoglobulin purification Bio-Adembeads PAG are particularly suitable for immunoglobulin purification from ascite, serum, tissue culture supernatants or other samples. After a short incubation time the immunoglobulins are eluted from the Bio-Adembeads PAG and the containing Ig supernatant is pipetted off for downstream applications.
Diameter 300 nm (CV max 20%)
Magnetic susceptibility approx. 40 emu/g
Specific surface area 10 m2/g
Iron oxide content approx. 70%
Binding capacity ~100µg/ml
Number of reactions 100 immunoprecipitations (Cat No 2004630) / 250 immunoprecipitations (Cat No 2004631)
Storage Supplied in storage buffer (Cat No 1010201) proclin 300 as a preservative Store at 2-8°C. The Bio-Adembeads PAG must be maintained in liquid during storage and all handling steps. Drying will result in reduced performance. Do not freeze the product.


  • Immunoprecipitation
  • Co-immunoprecipitation
  • Immunoglobulin purification
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