ROS indicator, Ex/Em = 495/529 nm. H2DCFDA [2′,7′-dichlorodihydrofluorescein diacetate] is a chemically reduced analogue of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent H2DCFDA is converted to the highly fluorescent 2',7'-dichlorofluorescein.
Learn More
MTT assay: determination of the cell viability through measurement of mitochondrial activity.
The mitochondrial activity of the cells is reflected by the conversion of the tetrazolium salt MTT into formazan crystals, which can be solubilised for homogenous measurement of optical density (OD) using a plate reader at 570 nm vs 690 nm. Learn More
MTT assay: determination of the cell viability through measurement of mitochondrial activity.
The mitochondrial activity of the cells is reflected by the conversion of the tetrazolium salt MTT into formazan crystals, which can be solubilised for homogenous measurement of optical density (OD) using a plate reader at 570 nm vs 690 nm. Learn More
MTT assay: determination of the cell viability through measurement of mitochondrial activity.
The mitochondrial activity of the cells is reflected by the conversion of the tetrazolium salt MTT into formazan crystals, which can be solubilised for homogenous measurement of optical density (OD) using a plate reader at 570 nm vs 690 nm. Learn More
ROS indicator, Ex/Em = 528/551 nm. Dihydrorhodamine 6G is the reduced form of rhodamine 6G, which is used as fluorescent mitochondrial dye. It is nonfluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 6G that accumulates in mitochondrial membranes. Dihydrorhodamine 6G is useful for detecting reactive oxygen species (ROS) including superoxide
Learn More
ROS indicator, Ex/Em = 495/529 nm. Carboxy-H2DCFDA [5-(and 6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate] is a chemically reduced analogue of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent Carboxy-H2DCFDA is converted to the highly fluorescent 5-(and 6)-carboxy-2',7'-dichlorofluorescein, with additional negative charges that impede its leakage out of the cell.
Learn More