DHR 123 ROS indicator
| Supplier |
|---|
| GeneCopoeia |
| Cat. no. |
| C261 |
| Kit size |
| 10 mg |
| Links |
Protocol  |
Features
- Fast and easy method
- Fluorescence reading
- Live cell assay
Generation of reactive oxygen species (ROS) at a controlled level is a feature of live cell function. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in damage of membrane lipids, proteins, and nucleic acids. Oxidative damage is associated with aging as well as many diseases. Probes for measuring ROS provide important tools for study oxidative stress related diseases.
Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. Dihydrorhodamine 123 itself is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide and peroxynitrite.
.| Format | 10 mg |
|---|---|
| Fluorescence reading | 507/529 nm |
- Detection of oxidative activities in viable cells
- Probe for ROS