HOT FIREPol 10x Master Mix B2 ((NH4)2SO4 based with 15 mM MgCl2)

Supplier
LabOmics Solis Biodyne
Cat. No
6310513
Pack size
1ml (500rxn)
Links
Data Sheet MSDS

Description:

• 10 x HOT FIREPol® Master Mix is a ready-to-use solution containing all reagents required for Hot Start PCR (except template, primers and water).

• Recommendations: Reaction setup at room temperature is highly recommended for HOT FIREPol® Master Mix. We recommend using 10 x HOT FIREPol® Master Mix in any PCR application that will be visualized by agarose gel electrophoresis and ethidium bromide staining.
• Shipping and Storage conditions: Routine storage: -20ºC Shipping and temporary storage for up to 1 month at room temperature or storage for up to 6 months at 2-8ºC has no detrimental effects on the quality of 10 x HOT FIREPol® Master Mix.

• Kit Composition:

• 10 x HOT FIREPol® Master Mix

• HOT FIREPol® DNA polymerase
• HOT FIREPol® 10 x Buffer B2

Tris-HCl, (NH4)2SO4 and detergent

• 15 mM MgCl2

1 x PCR solution – 1.5 mM MgCl2

• 2 mM dNTPs of each

1 x PCR solution – 200 μM dATP, 200 μM dCTP, 200 μM dGTP and 200 μM dTTP

• 100 mM MgCl2

• Hot Start PCR
• DHPLC
• TA cloning