M-MLV Reverse Transcriptase RNase H-

Supplier
LabOmics Solis Biodyne
Cat. No
670001
Pack size
10000units
Links
Data Sheet MSDS

Description:

• The M-MLV Reverse Transcriptase RNase H- is a genetically modified M-MLV Reverse Transcriptase which exhibits RNA or DNA dependent DNA polymerase, but lacks ribonuclease H activity. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase of full-length cDNA products.

Concentration:
200 U/µl
Unit definition:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol of dTTP into an acid insoluble form in 10 minutes at 37ºC.
Storage and Dilution buffer:
50% glycerol (v/v), 50 mM Tris-HCl pH 8.0 at 25ºC, 100 mM NaCl, 5 mM DTT, 1 mM EDTA, 0.1% NP-40.
Quality control:
Free of endo- and exodeoxyribonucleases, phosphatases and ribonuclease. Activity and stability tested in first strand cDNA synthesis.
Shipping and Storage conditions:
Routine storage: -20ºC. Shipping at room temperature has no detrimental effects on the quality of this reagent.

Kit Composition:
M-MLV Reverse Transcriptase RNase H-
5 x RT Reaction buffer 1(with MgCl2 and DTT)
0.25 M Tris-HCl, 0.5 M KCl, 30 mM MgCl2, 25 mM DTT
5 x RT Reaction buffer 2(Mg2+ and DTT free)
0.25 M Tris-HCl, 0.5 M KCl
25 mM MgCl2
20 mM MnCl2
100 mM DTT

cDNA synthesis
RNA analysis by primer extension
DNA labeling