miExpress™ Precursor miRNA Expression clones

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MicroRNA expression clones
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Vector based microRNA technology. Collection of miRNA expression clones available in a feline immunodeficiency virus (FIV) based lentiviral vector system. A RNA polymerase III type promoter (H1) is used to drive transcription of hairpin precursor miRNAs (
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GeneCopoeia
Product
miExpress™ Precursor miRNA Expression clones
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GeneCopoeia
GeneCopoeia information

Features

  • 650 human precursor miRNA expression clones are available which cover more than 95% of 678 known miRNAs in release 11.0 in miRBase


  • The expression cassettes of all miRNA expression clones have been fully sequenced


  • Lentiviral vector based expression constructs allow efficient infection based transduction of miRNAs into non-dividing and difficult to transfect target cells as well as transfection based transduction into conventional dividing cells


  • Stable cell line selection marker (neomycin) enables studies of the effect of both long term and transient expression regulation


  • Vector plasmid transfection and virus infection transduction efficiency are monitored by EGFP reporter protein.



MicroRNAs (or miRNAs) are small non-coding single-stranded RNA molecules that are transcribed from the genomes of eukaryotic organisms. They are highly conserved and usually 21~23 nucleotides in length. MiRNAs have been found to be involved in almost all cellular activities and functions. They regulate the expression of genes by binding to the 3’ untranslated regions (3’-UTRs) of targeted mRNAs. Irregularities in miRNA regulated gene expression have been found to be associated with cancers, cardiovascular disorders, and a variety of other diseases.

We are now offering: miExpress™ Precursor miRNA expression clones for 750 human, 450 mouse, and 270 rat genes and miTarget™ microRNA target sequence expression clones that cover 25,000 human and 20,000 mouse genes

Researchers can use these miRNA expression clones to study the expression regulation of corresponding genes/proteins. In combination with target sequence (3’-UTR) expression clones, which have luciferase as the reporter gene, researchers can also cross-validate. We also offer multiple sets of full length human and mouse expression ready ORF cDNA clones that can be used for gain-of-function studies of corresponding target genes.
We offer precursor miRNA expression clones constructed in a feline immunodeficiency virus (FIV) based lentiviral vector system. A RNA polymerase III type promoter (H1) is used to drive transcription of hairpin precursor miRNAs (approximately 150 nucleotides), which are then processed to generate mature miRNAs by the enzymes involved in RNAi machinery. A tracking/reporter gene EGFP is co-expressed with the neomycin selection gene under the control of CMV promoter and bicistronic IRES element, which provides a convenient way for monitoring miRNA expression, transfection and transduction efficiency. Specific gene regulation is achieved as the result of binding of RISC-miRNA complexes with the 3’UTR of the mRNAs of target genes (Fig. 1): 1) expression translation suppression and 2) mRNA cleavage, degradation, and translation reduction.



Fig. 1. Lentiviral vector based precursor miRNA expression clones and the mechanism of vector mediated miRNA gene regulation
  • Gene expression regulation. The regulatory and functional effect of the miRNAs can be studied individually or systematically using the whole set or a subset of miRNA expression constructs by transduction of precursor miRNAs into target cells either via transfection or viral infection
  • MiRNA target gene identification and validation. Together with GeneCopoeia’s miRNA target validation expression clones, miRNA expression constructs can be used to identify and verify their target genes
  • References:
  • Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T; "Identification of novel genes coding for small expressed RNAs"; Science. 294:853-858(2001).
  • Suh MR, Lee Y, Kim JY, Kim SK, Moon SH, Lee JY, Cha KY, Chung HM, Yoon HS, Moon SY, Kim VN, Kim KS; "Human embryonic stem cells express a unique set of microRNAs"; Dev Biol. 270:488-498(2004).
  • Dostie J, Mourelatos Z, Yang M, Sharma A, Dreyfuss G; "Numerous microRNPs in neuronal cells containing novel microRNAs"; RNA. 9:180-186(2003).
  • Michael MZ, O' Connor SM, van Holst Pellekaan NG, Young GP, James RJ; "Reduced accumulation of specific microRNAs in colorectal neoplasia"; Mol Cancer Res. 1:882-891(2003).
  • Kasashima K, Nakamura Y, Kozu T; "Altered expression profiles of microRNAs during TPA-induced differentiation of HL-60 cells"; Biochem Biophys Res Commun. 322:403-410(2004).
  • Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M, Weir DB, Choksi R, De Vita G, Frezzetti D, Trompeter HI, Hornung V, Teng G, Hartmann G, Palkovits M, Di Lauro R, Wernet P, Macino G, Rogler CE, Nagle JW, Ju J, Papavasiliou FN, Benzing T, Lichter P, Tam W, Brownstein MJ, Bosio A, Borkhardt A, Russo JJ, Sander C, Zavolan M, Tuschl T; "A mammalian microRNA expression atlas based on small RNA library sequencing"; Cell. 129:1401-1414(2007).
  • Lui WO, Pourmand N, Patterson BK, Fire A; "Patterns of known and novel small RNAs in human cervical cancer"; Cancer Res. 67:6031-6043(2007).
  • Marton S, Garcia MR, Robello C, Persson H, Trajtenberg F, Pritsch O, Rovira C, Naya H, Dighiero G, Cayota A; "Small RNAs analysis in CLL reveals a deregulation of miRNA expression and novel miRNA candidates of putative relevance in CLL pathogenesis"; Leukemia. 22:330-338(2008).
1. Identify the miRNA of interest:
You can browse the entire Precursor miRNA Clones Collection:
human
mouse
rat

Or gather as much as possible of the following information: Species, Precursor/Mature miRNA Name, Precursor/Mature miRNA Accession, Description or Catalog/product ID. You can also submit a sequence so we can search by Precursor or Mature Sequences homology (BLAST) and we will do the rest

Alternatively, you can use our search tool above or the search tool by GeneCoppoeia - Search miRNA clones by GeneCopoeia - and order the product ID result.

2. Fill out the order form:

and fill with requested information. Send it back to us by email or FAX. We will contact you shortly to confirm: sequences, vectors and related products


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