RNase-Free DNase I Kit

Supplier
Norgen Biotek
Cat. no.
1025710
Kit size
50
Links
Protocol Information Sheet

Features

• Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits
• Also includes protocol for digestion in-solution followed by RNA Clean-Up
• Guaranteed RNase-Free
• Includes Enzyme Incubation Buffer

Norgen’s RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen’s RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen’s RNA purification kits. Alternatively, after isolating total RNA using one of Norgen’s RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen’s RNA Clean-Up and Concentration Kit (Cat No 1023600), and the RNA can then be used in downstream applications. The amount of DNase I provided with this product is enough to perform 50 reactions.

Norgen’s RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination.  Should complete DNA removal be required for some applications, this enzyme and buffer set allows for convenient on-column or in-solution digestions.

RNase-Free DNase I	1 vial
Enzyme Incubation Buffer	6 ml