ROS Assay Kit for detection of ROS in live cells

Supplier
GeneCopoeia
Cat. no.
A057
Kit size
1000 assays
Links
Protocol

Features

• Fast and easy method

• Fluorescence reading

• Live cell assay

Generation of reactive oxygen species (ROS) at a controlled level is a feature of live cell function. Under conditions of oxidative stress, ROS production is dramatically increased, resulting in damage of membrane lipids, proteins, and nucleic acids. Oxidative damage is associated with aging as well as many diseases. Probes for measuring ROS provide important tools for study oxidative stress related diseases.

The Reactive oxygen species assay kit uses a unique fluorescent probe H2DCFDA to detect reactive oxygen species. H2DCFDA is chemically reduced and acetylated form of 2′,7′-dichloro- fluorescein (DCF) and is nonfluorescent and cell-permeant.   

Once H2DCFDA enters into cells, the acetate groups are removed by intracellular esterases to form H2DCF. H2DCF is well retained inside cells. Oxidation of H2DCF by reactive oxygen species yields fluorescent DCF. Reactive oxygen species can be detected by monitoring the increase in fluorescence with a flow cytometer, fluorometer, microplate reader, or fluorescence microscope, using excitation sources and filters appropriate for fluorescein (FITC). Because the dyes are susceptible to photo-oxidation, low light conditions should be used for fluorescence microscopy applications whenever possible.

• Detection of oxidative activities in viable cells

• Probe for ROS