Smart D-N-Adem-Kit for bacteria (gram plus or negative)

Supplier
Ademtech
Cat. No
2006131
Kit size
50 preps
Links
ademtech Protocol Information Sheet

Features

• Capture and direct amplification: go directly to PCR and Real-time PCR without elution step
• Enzymatic Digestion cocktail included within the kit : highly effective lyses method than mechanical method
• Method allows starting from Gram Positive and Gram Negative: Staphylococcus, Enterococcus, Shigella, Salmonella…
• Simple and fast: complete lysis at room temperature
• Higher recovery: no DNA lost during washing and elution step/strong>
• Greater sensitive compared with other technologies (spin column and organic extraction)
• Work with small amounts of samples: 10 bacterial medium or 1 colony
• Reproducible system: less sample variability

The Smart D-N-Adem-Kit is a new system which is especially designed for capture of gDNA and direct amplification on magnetic beads. Smart-Adembeads have specific and proprietary polymer on their surface, designed for DNA capture by electrostatic interactions and compatible with direct PCR and Real-Time PCR without the need to perform an elution step. Bacteria are mixed with the Lysis Buffer and Enzymatic Digestion Cocktail. Smart-Adembeads bind specifically to the gDNA. Proteins and other contaminants are then eliminated in the washing step. The purified gDNA bound to the Smart-Adembeads can be used directly for PCR and Real-Time PCR analyses. DNA isolation is achieved without phenol, ethanol, chloroform and ionic chaotropes; thus the purified gDNA bound to the Smart-Adembeads demonstrates improved downstream performance in PCR and Real-Time PCR. Unlike other purification systems, no elution of the gDNA is required, making it possible to maximize the amount of templates available for the reaction achieving greater sensitivity. This method makes this Kit ideal for processing small amount of samples where maximun gDNA recovery is critical.

• Add 50μl of Lysis Buffer to samples.
• Add 5μl of Enzymatic Digestion Cocktail and incubate at room temperature for recommended times
• Add 5μl of Proteinase K and 1μl of RNase and incubate at room temperature for 5 minutes
• Add 5μl of homogenized Smart-Adembeads
• Add 50 μl of Binding Buffer and incubate at room temperature for 1 minute.
• Place the tube on the magnet (also available from Labomics) for at least 1 minute and discard the supernatant.
• Wash twice with 100μl of Washing Buffer
• Place the tube on the magnet for at least 1 minute and discard the supernatant.
• Resuspend beads in 50μl of Amplification Buffer. Use the final solution directly for PCR and Real-Time PCR

Beads	250µL Smart-Adembeads
Buffers	5 mL Lysis Buffer	5 mL Binding Buffer	10 mL Washing Buffer	2.5 mL Amplification Buffer	Freeze-dryed Enzymatic Digestion Cocktail
Storage	Store at 2-8°C.

• capture of gDNA from bacteria Gram positive and Gram negative
• direct amplification by Standard PCR or RT-PCR