TERMIPol® DNA Polymerase
TERMIPol® DNA Polymerase is a thermostable DNA polymerase suited for MALDI-TOF mass spectrometry and other primer extension platforms. The enzyme has 5’→3’ polymerase activity and enhanced efficiency forincorporating unconventional nucleotides (ddNTPs and
£63 / €69
| Supplier |
|---|
| LabOmics Solis Biodyne |
| Cat. No |
| 6400500 |
| Pack size |
| 500units |
| Links |
Data Sheet MSDS |
Description:
- TERMIPol® DNA Polymerase is a thermostable DNA polymerase suited for MALDI-TOF mass spectrometry and other primer extension platforms. The enzyme has 5’?3’polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs and labeled ddNTPs).
Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.
Concentration: 5U/µL
Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acidinsoluble form in 30 minutes at 74ºC.
Storage and Dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Quality control: The enzyme is free of nicking and priming activities, exonucleases and non-specific endonucleases. Activity and stability tested via thermo cycling. The error rate per nucleotide per cycle is ~ 8 x 10-5. Estimated half life at 95ºC is 1.5 hours.
Shipping and Storage conditions: Routine storage: -20ºC. Shipping and temporary storage for up to 1 month at room
temperature has no detrimental effects on the quality of TERMIPol® DNA Polymerase.
Concentration: 5U/µL
Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acidinsoluble form in 30 minutes at 74ºC.
Storage and Dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Quality control: The enzyme is free of nicking and priming activities, exonucleases and non-specific endonucleases. Activity and stability tested via thermo cycling. The error rate per nucleotide per cycle is ~ 8 x 10-5. Estimated half life at 95ºC is 1.5 hours.
Shipping and Storage conditions: Routine storage: -20ºC. Shipping and temporary storage for up to 1 month at room
temperature has no detrimental effects on the quality of TERMIPol® DNA Polymerase.
Kit Composition:
TERMIPol® DNA Polymerase
10 x Reaction Buffer C (500 mM Tris-HCl pH 9.5 at 25ºC)
100 mM MgCl2
TERMIPol® DNA Polymerase
10 x Reaction Buffer C (500 mM Tris-HCl pH 9.5 at 25ºC)
100 mM MgCl2
MALDI-TOF mass spectrometry
Primer extension
Mass-array
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