Total RNA Purification Kit

In stock
SKU
1017200
245,00 €
Isolate total RNA from large RNA down to miRNA from as little as a single cell. No phenol, no chloroform.
Untitled Document
Supplier
Norgen Biotek
Cat. no.
1017200
Kit size
50
Links
Protocol
Information Sheet

Features

  • Fast and easy processing using rapid spin-column format
  • Isolate total RNA, from large rRNA down to microRNA (miRNA)
  • No phenol or chloroform extractions
  • Isolate high quality total RNA from a variety of sources
  • RNA can be isolated and detected from as little as a single animal cell

Norgen’s Total RNA Purification Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Lysis Solution. Ethanol is then added to the lysate, and the solution is loaded onto a spin-column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Buffer. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.
Column binding capacity 50 µg
Maximum column loading volume 600 µl
Average RNA yield HeLa cells (1x10exp6 cells): 15 µg E.coli (1x10exp9 cells): 50 µg S. cerevisiae (1x10exp7 cells): 30 µg
Isolate a Diversity of RNA Species.
Unlike most competitor´s kits (lanes C and D), Norgen´s Total RNA Purification Kit (lane A and B) allows for the isolation of all sizes of RNA, from the very large RNA down to the microRNA. Lane M is Norgen´s RNA Ladder. Total RNA, large and small RNA species, can be seen in lanes A and B (Norgen).















High Quality of Norgen's Isolated RNA Compared to a Competitor:
Total E. coli RNA analyzed on the Agilent® 2100 BioAnalyzer. Note the presence of small RNA species (red circle) in the samples Isolated via Norgen's kit and the absence of these RNA sp. in the comptitor RNA preparation.


















Great Sensitivity:
Total RNA was extracted from a decreasing number of 293 HEK cells followed by RT-PCR to detect isolated RNA. M is the marker lane.


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