TUNEL Andy Fluor™ 594 Apoptosis Detection Kit

In stock
SKU
A051
530,00 €
TUNEL Andy Fluor™ 594 Apoptosis Detection Kit- 50 Assays
Internucleosomal cleavage of DNA is a hallmark of apoptosis. DNA cleavage in apoptotic cells can be detected in situ in fixed cells or tissue sections using the terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) method. TUNEL is highly selective for the detection of apoptotic cells but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In the TUNEL assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Hapten-tagged dUTP (e.g. digoxigenin-dUTP or biotin-dUTP) can be detected using secondary reagents (e.g. anti-digoxigenin antibodies or streptavi­din) for fluorescence or colorimetric detection. Alternatively, fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry. The TUNEL Andy Fluor™ 594 Apoptosis Detection Kit contains dUTP conjugated to biotin and Streptavidin conjugated to bright and photostable Andy Fluor™ 594 red fluores­cent dye, for bright fluorescent TUNEL staining. Broad application: Suitable for FFPE tissue, frozen tissue and cells. High sensitivity: Can detect a low population of apoptotic cells. Bright apoptotic signal: Uses Andy Fluor™ dyes, resulting in a stable, non-photobleaching fluorescent signal. Specifications Platform: Fluorescence Microscopy Detection Method: Fluorescent Ex/Em: 590/615 nm Product Size: 50 assays Storage Conditions: -20 ℃, protect from light Shipping Condition: Dry ice Applications Cell apoptosis analysis.
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