TUNEL Assay Kit (HRP-DAB) 20 tests
TUNEL Assay Kit (HRP-DAB) 20 tests
Internucleosomal cleavage of DNA is a hallmark of apoptosis. DNA cleavage in apoptotic cells can be detected in situ in fixed cells or tissue sections using the terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling (TUNEL) method. TUNEL is highly selective for the detection of apoptotic cells but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In the TUNEL assay, TdT enzyme catalyzes the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Hapten-tagged dUTP (e.g. digoxigenin-dUTP or biotin-dUTP) can be detected using secondary reagents (e.g. anti-digoxigenin antibodies or streptavidin) for fluorescence or colorimetric detection. In this assay, it is horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP). Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis.
Product Size: 20 assays Storage Conditions: -20 ℃, protect from light Shipping Condition: Dry ice Applications Cell apoptosis analysis.
| Application | DNA Fragmentation |
| Detection method | HRP-DAB |
| Sample type | Paraffin section, frozen section, cell slide |
| Assay time | 3 hours |
| Detection instrument | Light microscope |
| Other reagents required | 4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),3% H2O2(E-IR-R115),Xylene，Ethanol，DNase I，PBS(E-IR-R187),ddH2O,Hematoxylin(E-IR-R120)，Neutral gum(E-IR-R118) |
| Storage | 2~8°C/-20°C. Refer to the Manual. |