ROS indicator, Ex/Em = 507/529 nm. Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. Dihydrorhodamine 123 itself is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide and peroxynitrite.
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Protein Carbonylation Assay Kit 100 tests (96 well plate)
Fast and easy method for the detection of Protein carbonylation in biological samples. Oxidative stress may cause reversible or irreversible changes in proteins. Such changes are meant to modulate protein function (redox regulation) or protect against irreversible damage that causes the inactive proteins to accumulate or become degraded.
The detection of carbonylated proteins is based on 2,4-dinitrophenylhydrazine (DNPH).
the reaction product (DNP) can be detected and quantified spectrophotometrycally at a typical absorption spectrum with a maximum at 365-375nm. Learn More
Superoxide indicator, Ex/Em = 300/610 nm. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (Ex/Em: 355/420nm) in cells, while the oxidized form ethidium has red fluorescence (Ex/Em: 300/610 nm) upon DNA intercalation.
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KineticBlue™ Fluorometric Cell Viability Assay Kit offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. Kinetic Blue™ detects cell viability by utilizing a blue and nonfluorescent dye resazurin, which is conv Learn More
Luc-Pair™ Duo-Luciferase HS Assay Kit (For High Sensitivity) 100 rxns
- High sensitivity The Luc-Pair™ Duo-Luciferase HS Assay Kit is the perfect choice for high sensitivity detection of Firefly luciferase (FLuc) and Renilla luciferase (Rluc) activiti…
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Luc-Pair™ Duo-Luciferase HS Assay Kit (For High Sensitivity) 300 rxns
- High sensitivity The Luc-Pair™ Duo-Luciferase HS Assay Kit is the perfect choice for high sensitivity detection of Firefly luciferase (FLuc) and Renilla luciferase (Rluc) activiti…
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Luc-Pair™ Duo-Luciferase HS Assay Kit (For High Sensitivity) 1000 rxns
- High sensitivity The Luc-Pair™ Duo-Luciferase HS Assay Kit is the perfect choice for high sensitivity detection of Firefly luciferase (FLuc) and Renilla luciferase (Rluc) activiti…
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Nitrite / Nitrate Determination 100 tests (96 well plate)
Fast and easy method for the detection of nitric oxide formation in biological samples. This method relies on an innovative cofactor technology allowing linearity over a broad range of concentration (over 100 µM) and reducing the handling and assay time
The kit is suitable for different sample types: plasma, serum, saliva, urine, cell lysate, tissue homogenate and culture medium.
In this assay, firstly, the nitrate is reduced to nitrite catalyzed by the nitrate reductase with cofactors and specific compounds to eliminate interferences.
The detection is based on the final product detection (diazonium compound, λmáx=540nm) obtained after nitrite reaction in several steps with sulfanilamide. Learn More
Luc-Pair™ Renilla Luciferase HS Assay Kit (For High Sensitivity) 100 rxns
- High sensitivity The Luc-Pair™ Renilla Luciferase Assay Kit is the perfect choice for high sensitivity detection of Renilla luciferase (RLuc) activity. The Luc-Pair™ Renilla Luciferase HS Assay Kit works great for many different eukaryotic cell types… Learn More
Luc-Pair™ Renilla Luciferase HS Assay Kit (For High Sensitivity) 300 rxns
- High sensitivity The Luc-Pair™ Renilla Luciferase Assay Kit is the perfect choice for high sensitivity detection of Renilla luciferase (RLuc) activity. The Luc-Pair™ Renilla Luciferase HS Assay Kit works great for many different eukaryotic cell types… Learn More