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Protein Carbonylation Assay Kit 100 tests (96 well plate) Fast and easy method for the detection of Protein carbonylation in biological samples. Oxidative stress may cause reversible or irreversible changes in proteins. Such changes are meant to modulate protein function (redox regulation) or protect against irreversible damage that causes the inactive proteins to accumulate or become degraded. The detection of carbonylated proteins is based on 2,4-dinitrophenylhydrazine (DNPH). the reaction product (DNP) can be detected and quantified spectrophotometrycally at a typical absorption spectrum with a maximum at 365-375nm. Learn More

Nitrite / Nitrate Determination 100 tests (96 well plate) Fast and easy method for the detection of nitric oxide formation in biological samples. This method relies on an innovative cofactor technology allowing linearity over a broad range of concentration (over 100 µM) and reducing the handling and assay time The kit is suitable for different sample types: plasma, serum, saliva, urine, cell lysate, tissue homogenate and culture medium. In this assay, firstly, the nitrate is reduced to nitrite catalyzed by the nitrate reductase with cofactors and specific compounds to eliminate interferences. The detection is based on the final product detection (diazonium compound, λmáx=540nm) obtained after nitrite reaction in several steps with sulfanilamide. Learn More

Thiol Quantification Assay KIT (-SH/S-S) 200 tests (96 well plate) The Thiol and Disulfide Assay Kit is recommended for estimations of oxidative stress levels in biological samples such as plasma. The method uses the measurement of oxidized and reduced thiols, using Ellman's reagent. This assay allows to determine both the native (naturally reduced) and total (chemically reduced) free thiols in each sample. The the read-out is made at 412 nm (OD) Learn More

ROS indicator, Ex/Em = 495/529 nm. H2DCFDA [2′,7′-dichlorodihydrofluorescein diacetate] is a chemically reduced analogue of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent H2DCFDA is converted to the highly fluorescent 2',7'-dichlorofluorescein. Learn More

Superoxide indicator, Ex/Em = 300/610 nm. Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (Ex/Em: 355/420nm) in cells, while the oxidized form ethidium has red fluorescence (Ex/Em: 300/610 nm) upon DNA intercalation. Learn More

ROS indicator, Ex/Em = 528/551 nm. Dihydrorhodamine 6G is the reduced form of rhodamine 6G, which is used as fluorescent mitochondrial dye. It is nonfluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 6G that accumulates in mitochondrial membranes. Dihydrorhodamine 6G is useful for detecting reactive oxygen species (ROS) including superoxide Learn More

ROS indicator, Ex/Em = 507/529 nm. Dihydrorhodamine 123 is the reduced form of rhodamine 123, which is a commonly used fluorescent mitochondrial dye. Dihydrorhodamine 123 itself is non-fluorescent, but it readily enters most of the cells and is oxidized by oxidative species or by cellular redox systems to the fluorescent rhodamine 123 that accumulates in mitochondrial membranes. Dihydrorhodamine 123 is useful for detecting reactive oxygen species including superoxide and peroxynitrite. Learn More

ROS indicator, Ex/Em = 495/529 nm. Carboxy-H2DCFDA [5-(and 6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate] is a chemically reduced analogue of fluorescein used as an indicator for reactive oxygen species (ROS) in cells. Upon cleavage of the acetate groups by intracellular esterases and oxidation, the nonfluorescent Carboxy-H2DCFDA is converted to the highly fluorescent 5-(and 6)-carboxy-2',7'-dichlorofluorescein, with additional negative charges that impede its leakage out of the cell. Learn More

ROS Assay Kit Detection of ROS in live cells 1,000 assays Learn More

Catalase Activity Assay Kit (100 tests) Learn More